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Home / Primary Antibodies

GFP Antibody (YFP) (F52058)

SKU : BHA17102004

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NSJ Bioreagents

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Mfr.No.	F52058, F52058-0.05ML, F52058-0.2ML
Clonality	Monoclonal
Description	Green fluorescent protein is one of the best visual reporters for monitoring gene expression in vivo and in situ. It is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or weste blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. NSJBio's anti-GFP antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. NSJBio's anti-GFP antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by weste blotting.
Formulation	In 1X PBS, pH 7.4, with 0.09% sodium azide
Host	Mouse
Immunogen Region	Purified His-tagged protein was used to produced this monoclonal YFP / GFP antibody.
Isotype	IgG1
Predicted Reactivity	GFP / YFP
Recombinant	No

Clone No	168AT1211

Format	Purified
Purification	Purified
Storage	Aliquot the GFP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Applications	WB, IF, ICC
Dilution	Weste blot: 1:4000,Immunofluorescence/Immunocytochemistry: 1:25

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17102004-1	F52058-0.05ML	0.05ml	$165		Add to Cart
BHA17102004-2	F52058-0.2ML	0.2ml	$389		Add to Cart

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