Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
Human plasma gelsolin was used as the immunogen for this Gelsolin antibody.
Isotype
IgG1
Predicted Reactivity
Human, rabbit
Reactivity
Human, Rabbit
Recombinant
No
Gene Id
2934
Clone No
GS-2C4
Format
Ascites
Purification
Ascites
Storage
After reconstitution, the Gelsolin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IHC-F
Dilution
Western blot: 0.5-1ug/ml,IHC (Paraffin): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml--formalin or acetone fixed tissues