GATA3 is a zinc finger transcription factor and plays an important role in promoting and directing cell proliferation, development, and differentiation in many tissues and cell types. GATA3 expression is primarily seen in breast and urothelial carcinomas. Therefore, GATA3 antibody can be useful in the identification of unknown primary carcinoma when carcinomas of the breast or bladder are a possibility.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 357-463 from the human protein were used as the immunogen for this GATA3 antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Optimal dilution of the GATA3 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P23771
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the GATA3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, FACS, WB
Description
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Western blot: 0.5-1ug/ml