It recognizes a 32kDa protein, which is identified as homodimer of LGALS13/Galectin 13 (also known as PP13). Galectins are a family of soluble beta-galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, Galectin 13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, Galectin-13 exists as a disulfide-linked homodimer. Galectin 13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-mateal blood-spaces. Screening for Galectin 13 in mateal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A human partial recombinant protein corresponding to amino acids 23-134 was used as the immunogen for the Galectin 13 antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Titering of the Galectin 13 antibody may be required for optimal performance.
Uniprot
Q9UHV8
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Galectin 13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, FACS, IF, IHC-P
Description
ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT