Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate. (UniProt)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A human recombinant partial protein corresponding to amin acids 372-472 was used as the immunogen for this recombinant FOXA1 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human, Rat
Note
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant FOXA1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P55317
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the recombinant FOXA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P, FACS
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Flow cytometry: 1-2ug/million cells