Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alteative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4282 recognizes recombinant human Follistatin 288, allowing for detection of FSH levels using various analysis methods. This antibody also works in a two site ELISA with FST/4281.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant human FS288 protein was used as the immunogen for the Follistatin antibody.
Isotype
Mouse IgG1
Species Reactivity
Human
Note
Optimal dilution of the Follistatin antibody should be determined by the researcher.
Uniprot
P19883
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Follistatin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, WB
Description
ELISA: use in combination with clone FST/4281 for two-site ELISA,Weste blot: 1-2ug/ml