Follistatin is a single-chain glycosylated protein that inhibits follicle stimulating hormone (FSH) release. Alternative splicing of Follistatin mRNA yields two isoforms, FS315 and FS288. FS288 is the main cell-surface form and binds to surface heparin sulphate proteoglycans. FST/4281 is a clone raised against recombinant Fst 288, and is used in combination, commonly as the capture, with antibody FST/4282 in a two site ELISA for the detection of Follistatin.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Human FS288 was used as the immunogen for the Follistatin antibody.
Isotype
Mouse IgG1
Species Reactivity
Human
Note
Optimal dilution of the Follistatin antibody should be determined by the researcher.
Uniprot
P19883
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Follistatin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, IHC-P
Description
ELISA: use in combination with clone FST/4282 for two-site ELISA,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT