Fluorescein-Labeled Mouse Anti-Human CD45 Monoclonal Antibody

Mfr.No.

A6712, A6713, A6714, A6716, 6610889, A6720, A6715, A6717, A3452, A3450

Description

The protein of CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.

Isotype

mouse IgG1

Kit Components

The main component of the product is fluorescein-labeled mouse anti-human CD45 antibody, pH7.2 PBS Buffer, 1% ProClin300.

Specificity	Clone	Subtype	Fluorescence	Λ Ex	Λ Em
CD45RA	HI100	IgG2b	FITC	488nm	525nm
			PE	488nm	575nm
			PerCP	488nm	670nm
			PE-Cy5	488nm	665nm
			PerCP-Cy5.5	488nm	695nm
			PE-Cy7	488nm	779nm
			APC	633nm	660nm
			APC-Cy7	633nm	779nm
			QB450	405nm	450nm
			QB500	405nm	500nm

Test Method

1. Take 2 tubes, add 100 μL of anticoagulated peripheral blood specimen, then add the recommended amount of the same fluorescein-labeled isotype control and this reagent respectively, and incubate at room temperature (18-25℃) for 15-30 minutes, protected from light.
2. Add 2mL of lysis separately, shake well and incubate for 10 minutes at room temperature (18-25℃) away from light.
3. Centrifuge at 300g for 5 minutes, discard the supernatant, add 2mL of PBS buffer to resuspend the cell precipitate, and shake well.
4. Centrifuge at 300g for 5 minutes, discard the supernatant, add 300μL PBS buffer to resuspend the cell precipitate, and incubate for 2 hours at 4°C, protected from light. If delayed detection (more than 2 hours) is required, use 300 μL of PBS buffer containing 1% paraformaldehyde to resuspend the cell precipitate and store at 2-8°C, protected from light, but the fixation time should not exceed 24 hours.

Usage

1. If the flow cytometer is not calibrated, or if the fluorescence compensation is insufficient and the gate is incorrectly positioned, false results may be generated.
2. To obtain the optimum signal-to-noise ratio, the antibodies have been calibrated, so please use the reagents strictly in accordance with their recommended dosage.
3. The data measured with this product are not directly comparable with similar data obtained by other methodological reagents or instruments.

Note

1. Materials such as biological samples, quality control/calibrators, and experimental waste should be handled as potentially infectious materials and disposed of using precautions that comply with regulations.
2. If the concentration of target cells in the sample is too low or too high, special treatment (such as diluting the blood sample or increasing the cell concentration) should be used before staining.
3. This product contains fluorescence. Do not come into direct contact with skin or food and be sure to wear gloves when handling.
4. This product is for research purposes only, not for in vitro diagnostic use.

Clone No	HI30

Storage	2~8℃, protected from light, DO NOT FREEZE.

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Combination SKU	Supplier No.	Size	Specification	Your price
BHA18400152-1	A6712	100T	FITC anti-human CD45	$80	Add to Cart
BHA18400152-2	A6713	100T	PE anti-human CD45	$100	Add to Cart
BHA18400152-3	A6714	100T	PerCP anti-human CD45	$250	Add to Cart
BHA18400152-4	A6716	100T	PE-Cy5 anti-human CD45	$220	Add to Cart
BHA18400152-5	6610889	100T	Percp-Cy5.5 anti-human CD45	$250	Add to Cart
BHA18400152-6	A6720	100T	PE-Cy7 anti-human CD45	$200	Add to Cart
BHA18400152-7	A6715	100T	APC anti-human CD45	$100	Add to Cart
BHA18400152-8	A6717	100T	APC-Cy7 anti-human CD45	$260	Add to Cart
BHA18400152-9	A3452	100T	QB450 anti-human CD45	$250	Add to Cart
BHA18400152-10	A3450	100T	QB500 anti-human CD45	$250	Add to Cart

Fluorescein-Labeled Mouse Anti-Human CD45 Monoclonal Antibody

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