Fluorescein-Labeled Mouse Anti-Human CD117 Monoclonal Antibody

Mfr.No.

A3072, A3073, A3075, A3077

Description

The protein of CD117 is a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein.

Isotype

mouse IgG1

Kit Components

The main component of the product is fluorescein-labeled mouse anti-human CD117 antibody, pH7.2 PBS Buffer, 1% ProClin300.

Specificity	Clone	Subtype	Fluorescence	Λ Ex	Λ Em
CD117	104D2	IgG1	FITC	488nm	525nm
			PE	488nm	575nm
			APC	633nm	660nm
			APC-Cy7	633nm	779nm

Test Method

1. Take 2 tubes, add 100 μL of anticoagulated peripheral blood specimen, then add the recommended amount of the same fluorescein-labeled isotype control and this reagent respectively, and incubate at room temperature (18-25℃) for 15-30 minutes, protected from light.
2. Add 2mL of lysis separately, shake well and incubate for 10 minutes at room temperature (18-25℃) away from light.
3. Centrifuge at 300g for 5 minutes, discard the supernatant, add 2mL of PBS buffer to resuspend the cell precipitate, and shake well.
4. Centrifuge at 300g for 5 minutes, discard the supernatant, add 300μL PBS buffer to resuspend the cell precipitate, and incubate for 2 hours at 4°C, protected from light. If delayed detection (more than 2 hours) is required, use 300 μL of PBS buffer containing 1% paraformaldehyde to resuspend the cell precipitate and store at 2-8°C, protected from light, but the fixation time should not exceed 24 hours.

Usage

1. If the flow cytometer is not calibrated, or if the fluorescence compensation is insufficient and the gate is incorrectly positioned, false results may be generated.
2. To obtain the optimum signal-to-noise ratio, the antibodies have been calibrated, so please use the reagents strictly in accordance with their recommended dosage.
3. The data measured with this product are not directly comparable with similar data obtained by other methodological reagents or instruments.

Note

1. Materials such as biological samples, quality control/calibrators, and experimental waste should be handled as potentially infectious materials and disposed of using precautions that comply with regulations.
2. If the concentration of target cells in the sample is too low or too high, special treatment (such as diluting the blood sample or increasing the cell concentration) should be used before staining.
3. This product contains fluorescence. Do not come into direct contact with skin or food and be sure to wear gloves when handling.
4. This product is for research purposes only, not for in vitro diagnostic use.

Clone No	104D2

Storage	2~8℃, protected from light, DO NOT FREEZE.

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Combination SKU	Supplier No.	Size	Specification	Your price
BHA18400215-1	A3072	100T	FITC anti-human CD117	$180	Add to Cart
BHA18400215-2	A3073	100T	PE anti-human CD117	$190	Add to Cart
BHA18400215-3	A3075	100T	APC anti-human CD117	$250	Add to Cart
BHA18400215-4	A3077	100T	APC-Cy7 anti-human CD117	$300	Add to Cart

Fluorescein-Labeled Mouse Anti-Human CD117 Monoclonal Antibody

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