FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.
Formulation
0.5mg/ml in PBS with 0.02% sodium azide and 50% glycerol
Host
Mouse
Immunogen Region
Amino acids DYKDDDDK were used as the immunogen for the FLAG Tag antibody.
Isotype
IgG2b
Predicted Reactivity
DYKDDDDK
Reactivity
DYKDDDDK
Recombinant
No
Clone No
1E6-
Format
Purified
Purification
Protein G affinity
Storage
After reconstitution, the FLAG Tag antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.