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Home / Primary Antibodies

Fascin Antibody (V2865IHC)

SKU : BHA17124440

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NSJ Bioreagents

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Mfr.No.	V2865IHC-7ML
Clonality	Monoclonal (mouse origin)
Description	Fascin/FSCN1 binds beta-catenin and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of FSCN1 in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. It localizes to actin-rich protrusions at the cell surface called filopodia. FSCN1 localizes to invadopodia, membrane protrusions formed at the adherent cell surface that facilitate extracellular matrix (ECM) invasion, this provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells since fascin expression is upregulated in a spectrum of cancers. (Wiki)
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Mouse
Immunogen Region	Full length recombinant human protein was used as the immunogen for the Fascin antibody.
Isotype	Mouse IgG2b, kappa
Species Reactivity	Human, Rat
Note	Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	Q16658

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Fascin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124440	V2865IHC-7ML	7 ml	$499		Add to Cart

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