This MAb specifically recognizes human estrogen receptor beta 5 (ERB5), a splice variant of the nuclear receptor hormone ER beta (ESR2), shown to exist in at least five isoforms (ERB1-ERB5). No cross-reactivity is seen with estrogen receptor beta 1 or 2 isoform. This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Amino acids LLSHVRHARYAP from the C-terminus of human Estrogen Receptor beta 5 were used as the immunogen for the Estrogen Receptor beta 5 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the Estrogen Receptor beta 5 antibody should be determined by the researcher.
Uniprot
Q92731
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Estrogen Receptor beta 5 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IF, WB, IHC-P
Description
Immunofluorescence: 2-4ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT