Estrogen receptor alpha (ER<font face=symbol>a</font>), also known as NR3A1, is one of two main types of estrogen receptor, a nuclear protein that is activated by the sex hormone estrogen. ERs are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. In humans, ER<font face=symbol>a</font> is encoded by the gene ESR1. It is mapped to 6q25.1. This gene is a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2/beta. Estrogen and its receptors are essential for sexual development and reproductive function, it also play a role in other tissues such as bone.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Human ERa partial recombinant protein (AA 425-595) was used as the immunogen for this Estrogen Receptor antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Subcellular Location
Nuclear
Gene Id
2099
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the Estrogen Receptor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml