Recognizes a protein of 110kDa, identified as Excision Repair Cross Complementing 1 (ERCC1). It is a mammalian nucleotide excision repair (NER) enzyme involved in repair of damaged DNA. ERCC1 is a homologous to RAD10 in Saccharomyces cerevisiae, which is required in mitotic intrachromosomal recombination and repair. ERCC1 is required in repair of cisplatin-induced DNA adducts and ultraviolet (UV)-induced DNA damage. High expression of ERCC1 has been linked to tumor progression in a variety of cancers including non-small cell lung cancer (NSCLC), squamous cell carcinoma of the head, ovarian cancer and esophageal cancer.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 191-281 was used as the immunogen for the ERCC1 antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human
Note
Optimal dilution of the ERCC1 antibody should be determined by the researcher.
Uniprot
P07992
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the ERCC1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.