Bifunctional aminoacyl-tRNA synthetase is an enzyme that in humans is encoded by the EPRS gene. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species. Alternative splicing has been observed for this gene, but the full-length nature and biological validity of the variant have not been determined.
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids R1298-Y1512) was used as the immunogen for the EPRS1 antibody.
Isotype
Rabbit IgG
Reactivity
Human
Recombinant
No
Uniprot
P07814
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format
Antigen affinity purified
Purification
Antigen affinity purified
Storage
After reconstitution, the EPRS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, IF, FACS
Dilution
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells
Reviews of EPRS1 Antibody / Proline--tRNA ligase (RQ6742)