EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. (UniProt)
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant human protein was used as the immunogen for the EpCAM antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Titering of the EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
P16422
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IHC-P, FACS, IF
Description
Flow cytometry: 1-2ug/million cells in 0.1ml,Weste blot: 2-4ug/ml,Immunofluorescence: 1-2 ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)