Epithelial cellular adhesion molecule (EpCAM), is also identified as epithelial specific antigen (ESA) and the 40kDa transmembrane epithelial glycoprotein EGP40. EpCAM is expressed on the baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. EpCAM antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Formulation
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host
Mouse
Immunogen Region
Small cell lung carcinoma cells were used as the immunogen for this antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human. Does not react with rat. Other species not known.
Note
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Format
PE Conjugate
Purity
Protein G affinity chromatography
Storage
Store the EpCAM antibody at 2-8°C. Conjugate is light-sensitive, protect from light.
Applications
FACS
Description
FACS: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood),Immunofluorescence: 1:50-1:100