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Home / Antibodies

EpCAM Antibody / Cytoplasmic domain (V2686SAF)

SKU : BHA17124182

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NSJ Bioreagents

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Mfr.No.	V2686SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A portion of amino acids 289-314 (cytoplasmic domain) was used as the immunogen for the EpCAM antibody.
Isotype	Mouse IgG2b, kappa
Species Reactivity	Human, Mouse, Rat, Dog, Cat
Note	Optimal dilution of the EpCAM antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P16422

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124182	V2686SAF-100UG	100 ug	$499		Add to Cart

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