EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
Small cell lung carcinoma cells were used as the immunogen for the EpCAM antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the EpCAM antibody should be determined by the researcher.
Uniprot
P16422
Format
Biotin Conjugate
Purity
Protein G affinity chromatography
Storage
Store the EpCAM antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).
Applications
FACS, IF, IHC-P
Description
Flow cytometry: 5ul per test per one million cells or 5ul per 100ul of whole blood,Immunofluorescence: 2-4ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml at RT