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Home / Primary Antibodies

EIF3E Antibody (RQ6619)

SKU : BHA17119033

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NSJ Bioreagents

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Mfr.No.	RQ6619
Cellular Localization	Cytoplasmic
Clonality	Polyclonal (rabbit origin)
Description	Eukaryotic translation initiation factor 3 subunit E is a protein that in humans is encoded by the EIF3E gene. The human homolog of EIF3E is located on chromosome region 8q22-q23. It is composed of 13 exons that span 45 kb of genomic DNA. EIF3E is the component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis ts localization/assembly. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). And the eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
Host	Rabbit
Immunogen Region	Recombinant human protein (amino acids A160-M249) was used as the immunogen for the EIF3E antibody.
Isotype	Rabbit IgG
Reactivity	Human, Mouse
Recombinant	No

Uniprot	P60228

Buffer	Lyophilized from 1X PBS with 2% Trehalose
Concentration	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Format	Antigen affinity purified
Purification	Antigen affinity purified
Storage	After reconstitution, the EIF3E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Applications	WB, IHC-P, FACS, Direct ELISA
Dilution	Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA17119033	RQ6619	100ug	$419		Add to Cart

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