EGF encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in EGFare the cause of hypomagnesemia type 4. Dysregulation of EGF has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Clonality
Polyclonal
Description
Rabbit Anti-EGF Polyclonal Antibody
Formulation
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen, Liquid solution
Host
Rabbit
Immunogen
Synthesized peptide derived from part region of human EGF protein at amino acid sequence of 1000-1060
Immunogen Region
Synthesized peptide derived from part region of human EGF protein at amino acid sequence of 1000-1060
Isotype
Rabbit IgG
Molecular Weight
130 KD
Others
The antibody detects endogenous levels of EGF
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Reactivity
Human
Tag
EGF
Note
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:200-1:500), ELISA (1:10000-1:20000).
Accession Number
P01133
Gene Id
1950, 1950
Concentration
1 mg/ml, 1 mg/ml
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen, The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen
Storage Buffer
PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol as stabilizer.
Storage Instructions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.