This mAb shows specificity to E. coli O157 in a simple ELISA. Escherichia coli are Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). The E. coli that cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenic E. coli, which are classified into the following four groups according to differences in the mode of pathogenicity; enteropathogenic E. coli (EPEC), enteroinvasive E. coli (EIEC), enterotoxigenic E. coli (ETEC) and enterohemorrhagic E. coli (EHEC). Although the identification of pathogenic E. coli requires verification of their pathogenicity, pathogenic E. coli often have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenic E. coli.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A crude sonicate of E. coli O157 was used as the immunogen for the E. coli O157 antibody.
Isotype
Mouse IgM, kappa
Species Reactivity
E. coli O157
Note
Optimal dilution of the E. coli O157 antibody should be determined by the researcher.
Format
Purified
Purity
PEG precipitation followed by dialysis in 1X PBS
Storage
Store the E. coli O157 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, IF
Description
ELISA: 1-5ug/ml for coating (order BSA/sodium azide-free format),Immunofluorescence: 1-2ug/ml