This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining patte in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Nuclei of Burkitt's cells were used as the immunogen for the dsDNA antibody.
Isotype
Mouse IgG3, kappa
Species Reactivity
Human
Note
Optimal dilution of the dsDNA antibody should be determined by the researcher.
Uniprot
Not Known
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the dsDNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, IHC-P
Description
Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)