Marker antibodies are useful in identification of organelles in cells, tissues, and biochemical preparations. This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. Double stranded (ds) DNA markers are useful tools in biology research and aid in the study of DNA behavior and characteristics.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Nuclei of Burkitt's cells were used as the immunogen for the Double Stranded DNA antibody.
Isotype
Mouse IgG3, kappa
Species Reactivity
Human
Note
Optimal dilution of the Double Stranded DNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
Not Known
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Double Stranded DNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
IHC-P
Description
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT