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Home / Primary Antibodies / Monoclonal Antibodies

Dityrosine Antibody, Clone 7D4 - SMC-520

SKU : BHA11905673

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StressMarq Biosciences Inc.

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Mfr.No.	SMC-520, SMC-520D, SMC-520D-A390, SMC-520D-A488, SMC-520D-A594, SMC-520D-ALP, SMC-520D-APC, SMC-520D-BI, SMC-520D-FITC, SMC-520D-HRP, SMC-520D-PCP, SMC-520D-RPE, SMC-520S
Alternative Names	Dityrosine Antibody, Dityrosine (DT) Antibody, DT Antibody, DY Antibody
Categories	Antibodies
Certificate Of Analysis	A 1:1000 dilution of SMC-520 was sufficient for detection of dityrosine in 1 ug of Dityrosine conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Clonality	Monoclonal
Description	Mouse Anti-Dityrosine (DT) Monoclonal IgG1
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Mouse
Gross Weight (kg)	1.4
Immunogen	Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Region	Synthetic Dityrosine conjugated to Keyhole Limpet Hemocyanin (KLH).
Isotype	IgG1
Launch Date	2017/03/23
Net Weight (g)	0.1
Reactivity	Species Independent
Recombinant	FALSE
Regulatory	RUO
Species Reactivity Medium	Species Independent

Target	Dityrosine
Clone No	7D4
Research Area	Cancer, Oxidative Stress, Protein Oxidation, Cell Signaling, Post-translational Modifications, Oxidation

Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.
Purification	Protein G Purified
Scientific Background	ROS such as hydrogen peroxide (H2O2), superoxide, and hydroxyl radicals can react with both the backbone and the side chains of proteins, leading to backbone cleavage and side-chain modifications, respectively. Peroxidases, UV radiation, and hydroxyl radicals catalyze the formation of tyrosyl radicals which then react to form cross-links between proteins (1). This produces dityrosine, a metabolically stable biomarker of protein oxidation (2).
Shipping Temperature	Blue Ice or 4°C
Specificity	Specific for dityrosine modified proteins. Does not cross-react with 3,5-dibromotyrosine or bromotyrosine modified proteins.
Storage	-20ºC
Storage Buffer	PBS pH 7.4, 50% glycerol, 0.09% Sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C
Trademark	MOLECULAR SIGNATURE

Applications	ELISA, WB, ICC/IF
Description	WB ; ICC ; IF ; ELISA
Dilution	WB (1:1000); ICC/IF (1:50); ELISA (1:1000); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Malencik, D. A., Anderson, S. R. (2003) Amino Acids. 25(3-4):233-247. 2. Giulivi, C., Davies, K. J. A. (2001) J Biol Chem. 276, 24129-24136.

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Combination SKU	Supplier No.	Conjugate	Size	Your price	Quantity
BHA11905673-1	SMC-520D	Unconjugated	100 ug	$409		Add to Cart
BHA11905673-2	SMC-520D-A390	ATTO 390	100 ug	$463		Add to Cart
BHA11905673-3	SMC-520D-A488	ATTO 488	100 ug	$462		Add to Cart
BHA11905673-4	SMC-520D-A594	ATTO 594	100 ug	$462		Add to Cart
BHA11905673-5	SMC-520D-ALP	Alkaline Phosphatase	100 ug	$455		Add to Cart
BHA11905673-6	SMC-520D-APC	APC	100 ug	$461		Add to Cart
BHA11905673-7	SMC-520D-BI	Biotin	100 ug	$458		Add to Cart
BHA11905673-8	SMC-520D-FITC	FITC	100 ug	$453		Add to Cart
BHA11905673-9	SMC-520D-HRP	HRP	100 ug	$449		Add to Cart
BHA11905673-10	SMC-520D-PCP	PerCP	100 ug	$461		Add to Cart
BHA11905673-11	SMC-520D-RPE	RPE	100 ug	$459		Add to Cart
BHA11905673-12	SMC-520S	Unconjugated	12 ug	$44		Add to Cart

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