Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant full-length human desmin protein was used as the immunogen for the Desmin antibody.
Isotype
Mouse IgG2b, kappa
Species Reactivity
Human, Dog, Cat, Horse, Cow
Note
Optimal dilution of the Desmin antibody should be determined by the researcher.
Uniprot
P17661
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the Desmin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.