Using both gain- and loss-of-function experiments, it was found that Xenopus Dbf4 inhibited canonical Wnt signaling. Depletion of endogenous Dbf4 did not disturb gastrulation movements or early organizer genes, but resulted in embryos with morphologically defective heart and eyes and suppressed cardiac markers. The function of Dbf4 in heart development appeared to be independent of its role in the cell cycle. Dbf4 interacted physically and functionally with Frodo, a context-dependent regulator of Wnt signaling.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
Recombinant human protein (amino acids Y295-S665) was used as the immunogen for the DBF4 antibody.
Isotype
IgG
Predicted Reactivity
Human
Reactivity
Human
Recombinant
No
Uniprot
Q9UBU7
Buffer
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the DBF4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.