Ten to forty percent of the patients with acquired immunodeficiency syndrome (AIDS) develop cytomegalovirus (CMV) infections. In some patients with AIDS, cytomegalovirus is detected in the bronchoalveolar lavage fluid (BALF), urine, and other specimens, even when there are no symptoms of cytomegalovirus disease. An indicator of active CMV infection is needed to facilitate the diagnosis of cytomegalovirus disease in patients with AIDS or HIV infection. Cytomegalovirus p65 antigen was detected in the leukocytes of both the peripheral blood and BALF during the early phase of CMV disease.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant Cytomegalovirus p65 protein was used as the immunogen for this PP65 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Cytomegalovirus
Note
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the PP65 antibody to be titered up or down for optimal performance.
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the PP65 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, ICC, IF
Description
ELISA (order BSA/sodium azide-free format for coating),Immunocytochemistry (Acetone- or paraformaldehyde-fixed): 0.5-1ug/ml for 30 min,Immunofluorescence: 0.5-1ug/ml