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Home / Primary Antibodies

Cytokeratin 8 /18 Antibody (V3113SAF)

SKU : BHA17124759

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NSJ Bioreagents

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Mfr.No.	V3113SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A Keratin preparation from human breast carcinoma MCF-7 cells was used as the immunogen for the Cytokeratin 8/18 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P05787, P05783

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Cytokeratin 8/18 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124759	V3113SAF-100UG	100 ug	$499		Add to Cart

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