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Home / Antibodies

Cytokeratin 10/13 Antibody (V3053SAF)

SKU : BHA17124662

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NSJ Bioreagents

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Mfr.No.	V3053SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Weste blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on tissue sections this mAb serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both coifying and non-coifying stratified epithelia and more differentiated cells of squamous carcinomas.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A cytoskeletal preparation extracted from human ectocervical epithelium was used as the immunogen for the Cytokeratin 10/13 antibody.
Isotype	Mouse IgG2a, kappa
Species Reactivity	Human
Note	Optimal dilution of the Cytokeratin 10/13 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P13645, P13646

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the Cytokeratin 10/13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	WB, FACS, IF, IHC-P
Description	Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Weste blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124662	V3053SAF-100UG	100 ug	$499		Add to Cart

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