Cytochrome P450 Oxidoreductase (CYPOR), also known as Cytochrome P450 Reductase, is a membrane-bound enzyme required for electron transfer to cytochrome P450 in the endoplasmic reticulumof the eukaryotic cell from a FAD- and FMN-containing enzyme NADPH: cytochrome P450 reductase. By Southern blot analysis of DNA isolated from a panel of 8 independent human-rodent somatic cell hybrids, Shephard et al.(1989) determined that CYPOR is encoded by a single gene located on 7pter-q22. By in situ hybridization to metaphase chromosomes, they refined the localization to 7q11.2. Cytochrome P450 oxidoreductase is a flavoprotein that donates electrons to all microsomal P450 enzymes, including the steroidogenic enzymes P450c17 (CYP17A1), P450c21 (CYP21A2), and CYP51A1 (Miller, 1986).
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the C-terminus of human Cytochrome P450 Oxidoreductase (DYIKKLMTKGRYSLDVWS) was used as the immunogen for this CYPOR antibody.
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Uniprot
P16435
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the CYPOR antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P, ICC
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml