This antibody recognizes a protein of 36kDa, identified as Cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression. The antibody is useful in identifying mantle cell lymphomas (Cyclin D1 positive) from CLL/SLL and follicular lymphomas (Cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Formulation
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host
Mouse
Immunogen Region
Human recombinant full length Cyclin D1 protein was used as the immunogen.
Isotype
Mouse IgG2a, kappa
Species Reactivity
Human, Monkey, Mouse and Rat. Other species not known.
Note
The dilution stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DCS-6 mAb to be titered up or down for optimal performance.
Format
PE Conjugate
Purity
Protein G affinity chromatography
Storage
Store the Cyclin D1 antibody at 2-8°C. Conjugate is light sensitive, store in the dark.
Applications
FACS, IF
Description
Flow Cytometry: 5ul/test/million cells or 5ul/test/100ul of whole blood,Immunofluorescence: 1:50-1:100 for 30 minutes at RT