Cue Domain-Containing Protein 2 is involved in ubiquitin-and proteasome-mediated degradation of progesterone receptor (PR, or PGR) and estrogen receptor(ER)-alpha. Hartz(2011) mapped the CUEDC2 gene to chromosome 10q24.32 based on an alignment of the sequence with the genomic sequence (GRCh37). Using coimmunoprecipitation analysis and protein pull-down assays, Zhang et al.(2007) confirmed that CUEDC2 interacted with the PRB isoform of PR. Mutation analysis revealed that the IF domain of PRB and the CUE domain of CUEDC2 were required for the interaction. CUEDC2 reduced PRB protein content and promoted progesterone-induced PRB degradation via the ubiquitin-proteasome pathway.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Rabbit
Immunogen Region
An amino acid sequence from the C-terminus of human Cue Domain-Containing Protein 2 (EAEEMKATYINLKPARKYRFH) was used as the immunogen for this CUEDC2 antibody (100% mouse homology).
Isotype
IgG
Predicted Reactivity
Human, Mouse, Rat
Reactivity
Human, Mouse, Rat
Recombinant
No
Uniprot
Q9H467
Format
Antigen affinity purified
Purification
Antigen affinity
Storage
After reconstitution, the CUEDC2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, IHC-P
Dilution
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml