Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. The beta subunit corresponds to residues 23-227. The alpha subunit corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A portion of amino acids 185-287 was used as the immunogen for the CLU antibody.
Isotype
Mouse IgG2c, kappa
Species Reactivity
Human
Note
Optimal dilution of the CLU antibody should be determined by the researcher.
Uniprot
P10909
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the CLU antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Applications
WB, IHC-P
Description
Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml