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Home / Antibodies

Clostridium difficile Toxin A Antibody (V8285)

SKU : BHA17117834

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NSJ Bioreagents

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Mfr.No.	V8285, V8285-20UG, V8285-100UG
Antigen	Clostridium difficile Toxin A
Clonality	Monoclonal
Description	This mAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
Formulation	0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Host	Mouse
Immunogen Region	C. difficile Toxin A was used as the immunogen for the Clostridium difficile Toxin A antibody.
Isotype	IgG1,kappa
Predicted Reactivity	Clostridium difficile
Reactivity	Clostridium difficile
Recombinant	No

Uniprot	P16154
Clone No	253/422

Concentration	0.2 mg/ml
Format	Purified
Purification	Protein G affinity chromatography
Storage	Store the Clostridium difficile Toxin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Applications	ELISA, IF
Dilution	ELISA (order BSA-free format for coating),Immunofluorescence: 1-2ug/ml

Product Manual	Data Sheet

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHA17117834-1	V8285-20UG	20ug	$219		Add to Cart
BHA17117834-2	V8285-100UG	100ug	$499		Add to Cart

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