This mAb recognizes mouse DNGR-1, which is also known as CLEC9A, which is a highly specific marker of the CD8a+ and the CD103+ DC subsets, as well as a receptor able to recognize a preformed signal exposed on necrotic cells. In peripheral blood, it is highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rat
Immunogen Region
RBL-2H3 cells expressing mouse CLEC9A fused to an HA epitope.
Isotype
Rat IgG1, kappa
Species Reactivity
Mouse
Note
Optimal dilution of the antibody should be determined by the researcher.
Uniprot
Q8RU4
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CLEC9A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, WB, IF
Description
Flow cytometry: 1-2ug/million cells in 0.1ml,Weste blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml