Recognizes protein of 192kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A recombinant N-terminal protein fragment from human Clathrin heavy chain was used as the immunogen for the Clathrin Heavy Chain antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the Clathrin Heavy Chain antibody should be determined by the researcher.
Uniprot
P53675, Q00610
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the Clathrin Heavy Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA
Description
ELISA (order BSA/sodium azide-free format for coating)