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Home / Primary Antibodies

Chromogranin A Antibody (V2099SAF)

SKU : BHA17123430

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NSJ Bioreagents

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Mfr.No.	V2099SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong chromogranin A antibody staining and absence of keratin antibody staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Human pheochromocytoma cells were used as the immunogen for this Chromogranin A antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human, Mouse, Rat
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Chromogranin A antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Format	Purified
Purity	Protein G purified monoclonal antibody
Storage	Store the Chromogranin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 0.1-0.2ug/ml for 30 min at RT

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17123430	V2099SAF-100UG	100 ug	$499		Add to Cart

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