Rabbit Anti-Human Choline Acetyltransferase Polyclonal

Mfr.No.	SPC-706, SPC-706D, SPC-706D-A390, SPC-706D-A488, SPC-706D-A594, SPC-706D-ALP, SPC-706D-APC, SPC-706D-BI, SPC-706D-FITC, SPC-706D-HRP, SPC-706D-PCP, SPC-706D-RPE, SPC-706S
Immunogen Species	Human
Alternative Names	CHAT_Human Antibody, Acetyl CoA choline O Acetyltransferase Antibody, Choline Acetylase Antibody, CLAT_Human Antibody, CMS1A Antibody
Categories	Antibodies
Cellular Localization	Cytoplasm, Cytosol, Mitochondrion, Nucleus
Certificate Of Analysis	A 1:1000 dilution of SPC-706 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
Clonality	Polyclonal
Description	Rabbit Anti-Human Choline Acetyltransferase Polyclonal
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host	Rabbit
Gross Weight (kg)	1.4
Immunogen	Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase, Homo Sapiens (Human)
Immunogen Region	Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
Launch Date	2015/10/01
Net Weight (g)	0.1
Reactivity	Mouse, Human
Recombinant	FALSE
Regulatory	RUO
Species	Homo Sapiens (Human)
Species Reactivity Medium	Mouse, Human

Swissprot	P28329
Accession Number	NP_001136401.1
Gene Id	1103
Target	Choline Acetyltransferase
Research Area	Neurotransmission, Neurotransmitter Receptors, Cell Signaling, Neurodegeneration, Alzheimer's Disease, Neuroscience

Buffer	PBS, 50% glycerol, 0.09% sodium azide
Concentration	1 mg/ml
Dilutions	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Purification	Peptide Affinity Purified
Scientific Background	Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).
Shipping Temperature	Blue Ice or 4°C
Specificity	Predicted molecular weight at ~82.5kDa. Observed molecular weights between 68-70kDa.
Storage	-20ºC
Storage Buffer	PBS, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Storage Temperature	-20°C

Applications	WB, ICC/IF
Description	WB ; ICC ; IF
Dilution	WB (1:1000); ICC/IF (1:100); optimal dilutions for assays should be determined by the user.

Product Manual	SDS
SDS

Reference	1. Houser C.R., Crawford G.D., Barber R.P., Salvaterra P.M., Vaughn J.E. (1983) Brain Research. 266(1): 97-119. 2. Karson C.N., Casanova M.F., Kleinman J.E.m Griffin W.S. (1993) Am J Psychiatry. 150: 454-459. 3. Baskins D.S., et al. (1999) Arch Neurol. 56: 1121-1123.

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Combination SKU	Supplier No.	Conjugate	Size	Your price
BHA11909325-1	SPC-706D	Unconjugated	100 ug	$331	Add to Cart
BHA11909325-2	SPC-706D-A390	ATTO 390	100 ug	$385	Add to Cart
BHA11909325-3	SPC-706D-A488	ATTO 488	100 ug	$384	Add to Cart
BHA11909325-4	SPC-706D-A594	ATTO 594	100 ug	$384	Add to Cart
BHA11909325-5	SPC-706D-ALP	Alkaline Phosphatase	100 ug	$377	Add to Cart
BHA11909325-6	SPC-706D-APC	APC	100 ug	$383	Add to Cart
BHA11909325-7	SPC-706D-BI	Biotin	100 ug	$380	Add to Cart
BHA11909325-8	SPC-706D-FITC	FITC	100 ug	$375	Add to Cart
BHA11909325-9	SPC-706D-HRP	HRP	100 ug	$371	Add to Cart
BHA11909325-10	SPC-706D-PCP	PerCP	100 ug	$383	Add to Cart
BHA11909325-11	SPC-706D-RPE	RPE	100 ug	$381	Add to Cart
BHA11909325-12	SPC-706S	Unconjugated	12 ug	$44	Add to Cart

Choline Acetyltransferase Antibody - SPC-706