Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin s disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
A CD32 peptide was used as the immunogen for this CDw75 antibody.
Isotype
Mouse IgM, kappa
Species Reactivity
Human
Note
Optimal dilution of the antibody should be determined by the researcher.
Format
Purified
Purity
PEG precipitation
Storage
Store the CDw75 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
FACS, IF, IHC-P
Description
Flow cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml