Recognizes a 34kDa protein, which is identified as cyclin dependent kinase 1 (cdk1) or p34cdc2 protein kinase. Cdc2 plays a crucial role during cell division and is most active during mitosis. It is predominantly localized in the nucleus. It is a serine/threonine kinase, which is activated by cyclin, presumably by de-phosphorylation of tyrosine residues. Activated Cdc2 performs specific functions during mitosis, including nuclear envelope breakdown and chromosome condensation.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Recombinant human protein was used as the immunogen for the CDC2 antibody.
Isotype
Mouse IgG2a, kappa
Species Reactivity
Human
Note
Optimal dilution of the CDC2 antibody should be determined by the researcher.
Uniprot
P06493
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CDC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
WB, IHC-P
Description
Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT,Weste blot: 1-2ug/ml