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Home / Antibodies

Cdc25B Antibody (R30622)

SKU : BHA17104235

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Mfr.No.	R30622
Clonality	Polyclonal
Description	Central to the onset of mitosis in all eukaryotic cells is the CDC2 protein kinase, the activity of which is negatively regulated by phosphorylation and positively activated by dephosphorylation. The latter function is carried out by a specific phosphatase, CDC25. At least 3 human<a href=../search_result.php?search_txt=cdc25>CDC25</a>genes code for the A, B, and C forms of CDC25.<a href=../search_result.php?search_txt=cdc25b>CDC25B</a>is mapped to 20p13. P38 kinase has a critical role in the initiation of a G2 delay after ultraviolet radiation. Inhibition of p38 blocks the rapid initiation of this checkpoint in both human and murine cells after ultraviolet radiation. In vitro, p38 binds and phosphorylates CDC25B at serines 309 and 361, and<a href=../search_result.php?search_txt=cdc25c>CDC25C</a>at serine-216; phosphorylation of these residues is required for binding to 14-3-3 proteins. In vivo, inhibition of p38 prevents both phosphorylation of CDC25B at serine-309 and 14-3-3 binding after ultraviolet radiation, and mutation of this site is sufficient to inhibit the checkpoint initiation. Regulation of CDC25B phosphorylation by p38 is a critical event for initiating the G2/M checkpoint after ultraviolet radiation.
Formulation	0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host	Rabbit
Immunogen Region	Amino acids 541-559 (DYRPMNHEAFKDELKTFRL-human) were used as the immunogen for this Cdc25B antibody.
Isotype	IgG
Predicted Reactivity	Human, Rat
Reactivity	Human, Rat
Recombinant	No

Uniprot	P30305

Format	Antigen affinity purified
Purification	Antigen affinity
Storage	After reconstitution, the Cdc25B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Applications	WB, IHC-P
Dilution	Weste blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml

Product Manual	Data Sheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17104235	R30622	100 ug	$419		Add to Cart

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