Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.Galaktionov K.I.,Cell 67:1181-1194(1991).The MGC Project Team;Genome Res. 14:2121-2127(2004).Wegener S., Eur. J. Cell Biol. 79:810-815(2000).
Host
Rabbit
Immunogen
Synthesized non-phosphopeptide derived from Human CDC25A around the phosphorylation site of serine 178 (Q-N-S(p)-A-P).
Raised In
Rabbit
Reactivity
Human
Regulatory
RUO
Relevance
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Galaktionov K.I.,Cell 67:1181-1194(1991). The MGC Project Team;Genome Res. 14:2121-2127(2004). Wegener S., Eur. J. Cell Biol. 79:810-815(2000).
Species
Homo Sapiens (Human)
Specificity
The antibody detects endogenous levels of total CDC25A protein.
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Pathway
Cell cycle Cellular senescence
Protein Families
MPI phosphatase family
Buffer
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Format
liquid
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.