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Home / Primary Antibodies

CD99 Antibody (V2709SAF)

SKU : BHA17124224

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NSJ Bioreagents

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Mfr.No.	V2709SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. It is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	Recombinant full-length human protein was used as the immunogen for the CD99 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the CD99 antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P14209

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the CD99 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	IHC-P
Description	Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at Room Temp

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17124224	V2709SAF-100UG	100 ug	$499		Add to Cart

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