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Home / Primary Antibodies

CD8A Antibody (V3471IHC)

SKU : BHA17125100

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NSJ Bioreagents

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Mfr.No.	V3471IHC-7ML
Clonality	Recombinant Rabbit Monoclonal
Description	CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4. It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Formulation	Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; For IHC use only
Host	Rabbit
Immunogen Region	Recombinant full-length human CD8a protein was used as immunogen for this recombinant CD8 antibody.
Isotype	Rabbit IgG, kappa
Species Reactivity	Human
Note	The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CD8 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P01732

Format	Purified
Purity	Protein A affinity chromatography
Storage	Store the recombinant CD8 antibody (with azide) at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Applications	IHC-P
Description	The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHA17125100	V3471IHC-7ML	7 ml	$499		Add to Cart

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