CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Rabbit
Immunogen Region
SU-DHL-4 lymphoma cells were used as the immunogen for the CD74 antibody.
Isotype
Rabbit IgG, kappa
Species Reactivity
Human
Note
Optimal dilution of the CD74 antibody should be determined by the researcher.
Uniprot
P044233
Format
Purified
Purity
Protein A/G affinity
Storage
Aliquot the CD74 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.