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Home / Primary Antibodies

CD73 Antibody (V7588SAF)

SKU : BHA17126751

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NSJ Bioreagents

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Mfr.No.	V7588SAF-100UG
Clonality	Monoclonal (mouse origin)
Description	CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
Formulation	1 mg/ml in 1X PBS; BSA free, sodium azide free
Host	Mouse
Immunogen Region	A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
Isotype	Mouse IgG1, kappa
Species Reactivity	Human
Note	Optimal dilution of the CD73 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Uniprot	P21589

Format	Purified
Purity	Protein G affinity chromatography
Storage	Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Applications	ELISA, FACS, IF, WB, IHC-P
Description	ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Product Manual	<a href="https://www.nsjbio.com/tds-pdf/cd73-antibody-nt5e-2646-human-protein-microarray-validated-v7588" target="_blank">Datasheet</a>

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SKU	Supplier No.	Size	Your price	Quantity
BHA17126751	V7588SAF-100UG	100 ug	$499		Add to Cart

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