CD70 belongs to the tumor necrosis factor (TNF) ligand family. It is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
The human WM-1 (Waldenstrom s macroglobulinemia) cell line was used as the immunogen for the CD70 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Optimal dilution of the CD70 antibody should be determined by the researcher.1. This mAb blocks the interaction between CD27 and CD70, and has been shown to inhibit T cell proliferation induced by dendritic cells.
Uniprot
P32970
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
Inhibition, ELISA, FACS, IF, IP, IHC-F
Description
ELISA (order BSA/sodium azide-free format for coating),Inhibits T cell proliferation induced by dendritic cells (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunoprecipitation: 1-2ug/500ug protein lysate,Immunohistochemistry (Frozen): 0.5-1ug/ml for 30 min at RT