This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Amino acids 18-135 from the human protein were used as the immunogen for the CD47 antibody.
Isotype
Mouse IgG2c, kappa
Species Reactivity
Human
Note
Optimal dilution of the CD47 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Uniprot
Q08722
Format
Purified
Purity
Protein G affinity chromatography
Storage
Store the CD47 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Applications
ELISA, WB, IHC-P
Description
ELISA (order BSA/sodium azide-free format for coating),Western blot: 1-2ug/ml for 60 min at RT,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT