CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Formulation
1 mg/ml in 1X PBS; BSA free, sodium azide free
Host
Mouse
Immunogen Region
Human B lymphocytes were used as the immunogen for the CD45 antibody.
Isotype
Mouse IgG1, kappa
Species Reactivity
Human
Note
Due to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.
Uniprot
P08575
Format
Purified
Purity
Protein G affinity chromatography
Storage
CD45 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Applications
IHC-P
Description
Immunohistology (FFPE): 0.5-1.0ug/ml for 30 min at RT